pSF-CMV-COOH-EKT-HA2 (OG70) C-Terminal Flu HA Tag Plasmid

Product Description

Adds a C terminal Influenza HA tag (frame 2) to a gene inserted into our main multiple cloning site. Also contains the CMV promoter to drive transcription.

$220.66
Product code: OG70
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Plasmid Information

Product Name: pSF-CMV-COOH-EKT-HA2 (discontinued)

Product Code: OG70

Size (bp): 4269 bp

Bacterial Antibiotic Selection: KanR

Origin and Compatibility: pUC high copy derived from pBR322

Bacterial Copy Number: 500-700 per cell

Promoter: Cytomegalovirus (CMV) immediate early promoter

Plasmid Purpose:

This vector adds a His tag that allows protein purification by binding to metal matrices such as nickel. The His tag will be positioned at the 3 prime end (C terminus) of a gene (expressed protein) in the primary MCS (NotI to XbaI). The His tag coding sequence contains six histidine residues that is downstream of a short glycine spacer and an enterokinase cleavage site (DDDDK) that can be used to remove the tag from a purified protein if required. It cleaves after the lysine residue.

Promoter Expression Level:

This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. For this reason we stock a range of other promoters that are compatible with this plasmid and are available on request.

Vector Sequence Files

Please select the file type you require. For reference most cloning programs will import a .gb (Genbank) file and will show all of the plasmids features automatically when downloaded and imported.

Transcription Termination:

This plasmid contains three alternative transcription terminators for mammalian bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.

Cloning in a Gene:

There is a start codon in the NcoI site can be removed by digestion with KpnI if required. The MCS for gene insertions extends from NotI to XbaI. There are built in Shine-Dalgarno and KOZAK sequences aligned with the start codon within the NcoI restriction site. If these are removed you will need to add a ribosomal entry site to the 5 prime end of your gene for optimal expression.

Intellectual Property Status

According to our IP-friendly policy this plasmid is sold free of reach-through rights and can be used to make commercial products. However the plasmid itself (or derivatives) cannot be sold.