pSF-CMV-HuIgG1 HC (OG527) Human IgG Heavy Chain Plasmid

Product Description

Human IgG heavy chain constant region expression vector where variable regions can be fused to the constant region to create your desired IgG heavy chain plasmid

$220.66
Product code: OG527
1
Buy From A Distributor
We have a worldwide distributor network allowing you to purchase your product locally in your currency

Plasmid Information

Product Name: pSF-CMV-HuIgG1_HC

Product Code: OG527

Size (bp): 5248 bp

Bacterial Antibiotic Selection: KanR

Origin and Compatibility: pUC high copy derived from pBR322

Bacterial Copy Number: 500-700 per cell

Promoter: Cytomegalovirus (CMV) immediate early promoter

Plasmid Purpose:

This expression plasmid contains the human IgG heavy chain constant region and has been designed to allow the seamless fusion of variable antibody fragments to create full length heavy chain expression constructs. The vector has been engineered to contain a BseRI restriction enzyme site upstream of the constant domain that when cleaved produces an overhang of the first two bases of the first codon of the constant region. This allows variable antibody fragments to be cloned upstream to create full length antibody genes. We also provide plasmids for human Kappa and Lambda light chain expression (termed pSF-CMV-HuKappa LC and pSF-CMV-HuLambda LC repectively). We also have a range of antibody expression plasmids for mouse and rat IgG heavy and light chains.

Promoter Expression Level:

This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. For this reason we stock a range of other promoters that are compatible with this plasmid and are available on request.

Transcription Termination:

This plasmid contains three alternative transcription terminators for mammalian bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.

Intellectual Property Status

According to our IP-friendly policy this plasmid is sold free of reach-through rights and can be used to make commercial products. However the plasmid itself (or derivatives) cannot be sold.