Mouse Lambda light chain constant region plasmid to allow the fusion of variable region coding sequences to produce antibody genes
Product Name: pSF-CMV-MuLambda
Product Code: OG565
Size (bp): 4573 bp
Bacterial Antibiotic Selection: KanR
Origin and Compatibility: pUC high copy derived from pBR322
Bacterial Copy Number: 500-700 per cell
Promoter: Cytomegalovirus (CMV) immediate early promoter
This cloning vector contains the mouse (Mus musculus) lambda antibody light chain constant region and is used to create full length light chain antibody expression constructs. This is possible because the constant region is positioned downstream of a restriction site called BseRI which when cut will produce an overhang from the first two nucleotudes of the first codon of the constant region coding sequence. This allows variable regions to be inserted seamlessly by adding a BseRI site to the end of the variable region in the opposing direction.Promoter Expression Level:
This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. For this reason we stock a range of other promoters that are compatible with this plasmid and are available on request.
This plasmid contains three alternative transcription terminators for mammalian bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.
According to our IP-friendly policy this plasmid is sold free of reach-through rights and can be used to make commercial products. However the plasmid itself (or derivatives) cannot be sold.