pSF-CMV-SnapFusion (OG603) CMV Mammalian SnapFusion Expression Plasmid

Product Description

Mammalian vector designed for the insertion of genes that have been configured for fusion peptide tag range, but in this plasmid there is no tag to allow normal un-taggged expression.

$220.15
Product code: OG603
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Plasmid Information

Product Name: pSF-CMV-SnapFusion

Product Code: OG603

Size (bp): 4255 bp

Bacterial Antibiotic Selection: KanR

Origin and Compatibility: pUC high copy derived from pBR322

Bacterial Copy Number: 500-700 per cell

Promoter: Cytomegalovirus (CMV) immediate early promoter

Plasmid Purpose:

This vector is a standard mammalian expression plasmid with the exception that it has been modified to allow the direct cloning of gene fragments that have been designed to fit with any of our peptide tag plasmid range. This can be useful if you have created a peptide fusion but you would also like to create a non-tagged gene variant. The multiple cloning site contains two BsgI and BseRI sites that produce overhangs that are compatible with gene designed using our SnapFusion primer designer (see the cloning tab for this product).

Promoter Expression Level:

This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. For this reason we stock a range of other promoters that are compatible with this plasmid and are available on request.

Transcription Termination:

This plasmid contains three alternative transcription terminators for mammalian bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.

Intellectual Property Status

According to our IP-friendly policy this plasmid is sold free of reach-through rights and can be used to make commercial products. However the plasmid itself (or derivatives) cannot be sold.