pSF-pA-MinProm-SEAP (OG248) Minimal Promoter SEAP Plasmid

Product Description

HSV TK minimal promoter Alkaline phosphatase reporter plasmid. The minimal promoter also contains a poly A site upstream to reduce background.

$221.58
Product code: OG248
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Plasmid Information

Product Name: pSF-pA-MinProm-SEAP

Product Code: OG248

Size (bp): 5572 bp

Bacterial Antibiotic Selection: KanR

Origin and Compatibility: pUC high copy derived from pBR322

Bacterial Copy Number: 500-700 per cell

Promoter: HSV TK Minimal Promoter

Plasmid Purpose:

This plasmid contains a multiple cloning site that is upstream of a minimal promoter that is upstream of the human secreted alkaline phosphatase (SEAP) reporter gene. This plasmid can be used to insert specific DNA sequences that will endow the minimal promoter with specific biochemical properties such as tissue specificity or transcription factor specific activation.

Vector Sequence Files

Please select the file type you require. For reference most cloning programs will import a .gb (Genbank) file and will show all of the plasmids features automatically when downloaded and imported.

Transcription Termination:

This plasmid contains three alternative transcription terminators for mammalian bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.

Multiple Cloning Site Notes:

There is a start codon in the NcoI site in the MCS that can be removed by digestion with KpnI if required. The MCS for gene insertions extends from NotI to XbaI but there are built in Shine-Dalgarno and Kozak ribosomal entry site sequences aligned with the start codon in the NcoI site. The ClaI and NheI sites have other functions and allow the insertion of sequences from our other catalogue plasmids (such as IRES sequences and second promoters).

The BsgI and BseRI restriction sites in the plamsid cleave within the stop codon in the XbaI site in the MCS. This can allow retrospective fusion of coding sequences to our peptide tag range if your genes stop codon is positioned here.

Intellectual Property Status

According to our IP-friendly policy this plasmid is sold free of reach-through rights and can be used to make commercial products. However the plasmid itself (or derivatives) cannot be sold.