T7 promoter plasmid containing the EMCV IRES sequence to allow the production of mRNA using cap-independent expression in mammalian cells.
Product Name: pSF-T7-EMCV
Product Code: OG185
Size (bp): 4378 bp
Bacterial Antibiotic Selection: KanR
Origin and Compatibility: pUC high copy derived from pBR322
Bacterial Copy Number: 500-700 per cell
Promoter: T7 Polymerase Promoter
This vector allows transcription of un-polyadenylated and un-capped RNA using the T7 bacteriophage polymerase. This plasmid also contains the internal ribosome entry site (IRES) from Encephalomyocarditis virus (ECMV) between the promoter and the multiple cloning site (MCS). It is designed to be used to express genes using eukaryotic T7 expression systems such as those used for the recovery of a variety of viruses. This vector is not designed for the expression of two proteins from the same mRNA. For this purpose we have designed pSF-CMV-MCS-IRES-PciI where the PciI site contains a start codon in the correct position for the IRES to express efficiently. In this vector there is an NcoI site (which contains an ATG start codon) in the correct position to enable efficient expression of a gene in the MCS.
Please select the file type you require. For reference most cloning programs will import a .gb (Genbank) file and will show all of the plasmids features automatically when downloaded and imported.
This plasmid contains three alternative transcription terminators for mammalian bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.
According to our IP-friendly policy this plasmid is sold free of reach-through rights and can be used to make commercial products. However the plasmid itself (or derivatives) cannot be sold.