Plasmid Production Services

Oxford Genetics produces high quality plasmid DNA for a variety of research applications. We are able to manufacture at a range of scales and meet the rapid turnaround times demanded by our clients. 

 

Scale

Midiprep

Maxiprep

Megaprep

Gigaprep

Yield – High Copy Number Plasmid

75 to 100 µg

250 to 500 µg

1 to 2.5 mg

5 to 10 mg

Yield – Low Copy Number Plasmid

20 to 100 µg

100 to 500 µg

0.3 mg to 1.5 mg

1 to 2.5 mg

Turnaround Time

1 – 2 weeks

1 – 2 weeks

1 – 2 weeks

2 weeks

Methodology

Alkaline Lysis and Anion-Exchange Chromatography Purification

Alkaline Lysis and Anion-Exchange Chromatography Purification

Alkaline Lysis and Anion-Exchange Chromatography Purification

Alkaline Lysis and Anion-Exchange Chromatography Purification

Analysis

UV spectrophotometry at 260 nm and restriction digeston 

UV spectrophotometry at 260 nm and restriction digeston 

UV spectrophotometry at 260 nm and restriction digeston 

UV spectrophotometry at 260 nm and restriction digeston 

Amount of plasmid DNA required from customer

1 - 5 µg

1 - 5 µg

1 - 5 µg

1 - 5 µg

 

 

Project Outline

A typical plasmid production project consists of the following phases. The actual plasmid DNA yield will vary depending on the plasmid copy number, type and size, the bacterial strain and the growth of that in culture.

 

 

 

Additional Services

In addition to the production of plasmid DNA, the following additional services are also available.

 

Genetic Analysis

PCR/Sanger sequencing of plasmid DNA

Plasmid Optimization

Expression construct optimization, including introduction of alternative promoter sequences; inclusion of optimal sequences in the 5’ UTR, 3’ UTR and poly-adenylation regions; choice of Kozak and stop codons; inclusion of exogenous genes to increase protein yield

Animal Component Free Production

When required plasmid production can be carried out using a fully-traceable, animal component free process.