Peptide Tags

We stock the world's largest collection of plasmids encoding functional peptide tags. The tags are positioned automatically at either the N or C terminus when you insert your gene of interest into the MCS, using a range of unique enzyme sites.

We currently offer plasmids containing the following tags, for expression in mammalian, yeast or bacterial cells:Mamma Tag Fig

  1. Histidine (6 His)
  2. Histidine (10 His)
  3. Influenza HA
  4. C-Myc
  5. FLAG
  6. Strep
  7. V5
  8. Glutathione-S-transferase (GST)
  9. Maltose binding protein (MBP)
  10. T7
  11. S-Tag

For more information about these tags see our peptide tag guide.

Protease Cleavage Sites

All of our peptide tags are available with five different protease cleavage sites including: Enterokinase (EKT) / Rhinovirus 3C (PreScission) / Tobacco Etch Virus (TEV)/ Thrombin (Throm)/ Factor Xa. For more information on these enzyme sites, please see our cleavage tag guide.

The plasmid structure shown contains a 6His affinity tag and a V5 epitope tag, both upstream of a TEV cleavage site, all positioned upstream of the MCS. When you insert your gene of interest into the MCS, both the functional tags and the cleavage site will be positioned at the N terminal of the expressed protein. This will allow you to produce your protein, purify and characterise it, and simply remove the tags (using TEV proteolysis) if you wish. We have literally hundreds of variations on this structure, to meet your precise requirements.

If you want a plasmid that provides functional tags alongside a secretory tag (signal peptide), to engineer secretion of your protein product from mammalian cells, please check out the products onhttp:///dna mammalian secretory tags page.

We also provide plasmids encoding reporter gene tags (fusion proteins) including Renilla and Firefly luciferase, Beta Galactosidase, Alkaline Phosphatase, Chloramphenicol Acetyl transferase and a range of fluorescent reporters. Plehttp:///fusion our Reporter Fusion Proteins page. 

Please use the Tables below to find the mammalian tag plasmid you require. Alternatively you can search using the Plasmid Search tool, or browse our tags catalogues using the buttons at the bottom of this page. If in doubt, please contact us.

Plasmids Introducing Functional Tags onto Your Gene of Interest

 

 

N Terminal Tag Plasmids in Blue

C Terminal Tag Plasmids in Red

Protease Cleavage Site

For removal of tag after purification or analysis of your protein

 

For expression in mammalian cells

For expression in bacterial cells

 

none

EKT

TEV

FXa

Thrombin

3C

none

EKT

TEV

FXa

Thrombin

3C

 

 

Epitope Tags

V5

OG3422

OG3202

OG3411

OG3194

OG3404

OG3191

OG3400

OG3192

OG3401

OG3196

OG3405

OG3316

OG3178

OG3306

OG3167

OG3297

OG3508

OG3294

OG3505

OG3295

OG3506

OG3300

OG3511

 

Ha

OG3215

OG3425

OG3200

OG3409

OG1127

OG1128

OG1067

OG1068

OG1087

OG1088

OG1145

OG1146

OG3319

OG3181

OG3304

OG3515

OG2774

OG2775

OG2708

OG2709

OG2730

OG2731

OG2792

OG2793

 

C-Myc

OG3214

OG3424

OG3199

OG3408

OG1126

OG1125

OG1065

OG1066

OG1085

OG1086

OG1143

OG1144

OG3318

OG3180

OG3303

OG3514

OG2772

OG2773

OG2706

OG2707

OG2728

OG2729

OG2790

OG2791

 

S-Tag

OG3219

OG3429

OG1163

OG1164

OG1135

OG1136

OG1075

OG1076

OG1095

OG1096

OG1153

OG1154

OG3323

OG3185

OG2812

OG2813

OG2782

OG2783

OG2716

OG2717

OG2738

OG2739

OG2800

OG2801

 

T7

OG3218

OG3428

OG1161

OG1162

OG1133

OG1134

OG1073

OG1074

OG1093

OG1094

OG1151

OG1152

OG3322

OG3184

OG2810

OG2811

OG2780

OG2781

OG2714

OG2715

OG2736

OG2737

OG2798

OG2799

 

FLAG

OG3213

OG3423

OG3198

OG3407

OG1123

OG1124

OG1063

OG1064

OG1083

OG1084

OG1141

OG1142

OG3317

OG3179

OG3302

OG3513

OG2770

OG2771

OG2704

OG2705

OG2726

OG2727

OG2788

OG2789

 

Affinity Tags

MBP

OG3220

OG3430

OG1165

OG1166

OG3195

OG3403

OG1077

OG1078

OG1097

OG1098

OG1155

OG1156

OG3324

OG3186

OG2816

OG2817

OG3298

OG3509

OG2720

OG2721

OG2742

OG2743

OG2804

OG2805

 

GST

OG3216

OG3426

OG3201

OG3410

OG1129

OG1130

OG1069

OG1070

OG1089

OG1090

OG1147

OG1148

OG3320

OG3182

OG3305

OG3516

OG2776

OG2777

OG2710

OG2711

OG2732

OG2733

OG2794

OG2795

 

Strep

OG3217

OG3427

OG1159

OG1160

OG1131

OG1132

OG1071

OG1072

OG1091

OG1092

OG1149

OG1150

OG3321

OG3183

OG2808

OG2809

OG2778

OG2779

OG2712

OG2713

OG2734

OG2735

OG2796

OG2797

 

6His

OG3211

OG3421

OG3197

OG3406

OG1121

OG1122

OG1061

OG1062

OG1081

OG1082

OG1139

OG1140

OG3329

OG3517

OG2814

OG2815

OG3299

OG3510

OG2718

OG2719

OG2740

OG2741

OG2802

OG2803

 

10His

OG3210

OG3420

OG1157

OG1158

OG1119

OG1120

OG1059

OG1060

OG1079

OG1080

OG1137

OG1138

OG3315

OG3177

OG3301

OG3512

OG2768

OG2769

OG2702

OG2703

OG2724

OG2725

OG2786

OG2787

 

Dual

Tags

 

V5 & 6His

OG3203

OG3209

OG3412

OG3419

OG3207

OG3417

OG3204

OG3413

OG3205

OG3415

OG3208

OG3418

 

 

 

 

 

 

 

 

HA & 6His

OG1179

OG1267

OG1180

OG1268

OG1235

OG1236

OG1187

OG1188

OG1203

OG1204

OG1251

OG1252

 

 

 

 

 

 

 

 

c-Myc & 6His

OG1265

OG1177

OG1266

OG1178

OG1233

OG1234

OG1185

OG1186

OG1201

OG1202

OG1249

OG1250

 

 

 

 

 

 

 

 

S-Tag & 6His

OG1171

OG1275

OG1172

OG1276

OG1243

OG1244

OG1195

OG1196

OG1211

OG1212

OG1259

OG1260

 

 

 

 

 

 

 

 

T7 & 6His

 

OG1169

OG1273

OG1170

OG1274

OG1241

OG1242

OG1193

OG1194

OG1209

OG1210

OG1257

OG1258

 

 

 

 

 

 

 

 

FLAG & 6His

OG1175

OG1263

OG1176

OG1264

OG1231

OG1232

OG1183

OG1184

OG1199

OG1200

OG1247

OG1248

 

 

 

 

 

 

 

 

MBP & 6His

OG1173

OG1277

OG1174

OG1278

OG1245

OG1246

OG1197

OG3414

OG1213

OG1214

OG1261

OG1262

 

 

 

 

 

 

 

 

GST & 6His

OG1181

OG1269

OG1182

OG1270

OG1237

OG1238

OG1189

OG1190

OG1205

OG1206

OG1253

OG1254

 

 

 

 

 

 

 

 

Strep & 6His

OG1167

OG1271

OG1168

OG1272

OG1239

OG1240

OG1191

OG1192

OG1207

OG1208

OG1255

OG1256