Bacterial secretory tag properties and uses:

Oxford Genetics offers a range of secretory tags for use in bacterial cells in easy-to-clone formats.

Our product range

Oxford Genetics provides versatile cloning plasmids containing the bacterial secretory tags listed below. dsba Secretory tag1

  • OmpA (outer membrane protein A)
  • OmpC (outer membrane protein C)
  • OmpT (outer membrane protein T)
  • PelB (pectate lyase B)
  • TorA (tor operon component)
  • TorT (tor operon component)
  • MalE
  • Dsba (dithiol-disulfide oxidoreductase)
  • gIII (M13 phage minor coat protein)
  • PhoA (bacterial alkaline phosphatase)
  • Sufl

Simply inserting your gene within the MCS will introduce the secretory tag onto the N terminus, and allow it to be automatically removed by cleavage during secretion. The structure of a plasmid encoding the Dsba secretory tag is shown in the diagram.

Simple constructs

Each of these proteins is provided in a vector allowing N-terminal fusion with your gene of interest by inserting it into the MCS. The tag will be automatically removed by cleavage during secretion.

The vectors usually also include kanamycin resistance to enable simple selection of transfected bacterial cells.

Simple secretory tag plasmids for use in bacterial cells

Tag

OmpA

OmpC

OmpT

PelB

TorA

TorT

Product code

OG3099

OG3160

OG3161

OG3157

OG3164

OG3165

Tag

MalE

Dsba

gIII

PhoA

Sufl

 

Product code

OG3166

OG3158

OG3159

OG3162

OG3163

 

Constructs containing Secretory Peptides and Additional Functional Tags

We have had particular success using the OmpA and PelB secretory tags, and we provide the world’s most extensive range of products using those particular tags in a very broad range of formats.

For example both OmpA and PelB-containing plasmids are provided with additional epitope and affinity tags, allowing simple identification and/or purification of your expressed proteins. These tags include hexahistidine and decahistidine, V5, C-Myc, T7, haemagglutinin and several others. Please see the ‘view products’ button below, or use the Plasmid Finder on the Home Page.

Constructs containing Secretory Peptides, Additional Functional Tags and Protease Cleavage Sites Enabling Removal of Functional Tags After Protein ProductionOmpA-EKT secretory1

Many of our plasmids that contain functional tags (eg. Epitope tags, in addition to secretory tags) also contain simple protease cleavage sites. This allows you to remove the tag, if you so desire, following protein production and identification/purification. The structure of a plasmid encoding the OmpA secretory tag, with Flag tag and EKT cleavage site is shown.

We sell a broad range of cleavage sites, including sites for EKT, Factor Xa, Thrombin, TEV, 3C (Prescission) etc Because we provide a large number of products with three components (secretory tag, additional functional tag and cleavage site) we have tabulated them below to help you identify which plasmid you need.

For simplicity, all of our tags are positioned upstream of the MCS and will be included at the N terminal of your inserted protein sequence. This should be sufficient for all needs, however do contact us if you require C terminal tags, as these are very easy for us to provide.

 

Secretory tag plasmids with additional functional tags, for use in bacterial cells

Secretory Tag: OmpA

Functional Tag

T7

V5

FLAG

Strep

C-Myc

6-His

HA

S-Tag

 

 

Protease Cleavage Site

EKT

OG3073

OG3049

OG3067

OG3085

OG3055

OG3043

OG3061

OG3079

Thr

OG3075

OG3051

OG3069

OG3087

OG3057

OG3045

OG3063

OG3081

FXa

OG3076

OG3052

OG3070

OG3088

OG3058

OG3046

OG3064

OG3082

TEV

OG3072

OG3048

OG3066

OG3084

OG3054

OG3042

OG3060

OG3078

3C

OG3077

OG3053

OG3071

OG3089

OG3059

OG3047

OG3065

OG3083

-

OG3095

OG3092

OG3098

OG3097

OG3093

OG3090

OG3094

OG3096

Secretory Tag: PelB

Functional Tag

T7

V5

FLAG

Strep

C-Myc

6-His

HA

S-Tag

 

Protease Cleavage Site

EKT

OG3131

OG3107

OG3125

OG3143

OG3113

OG3101

OG3119

OG3137

Thr

OG3133

OG3109

OG3127

OG3145

OG3115

OG3103

OG3121

OG3139

FXa

OG3134

OG3110

OG3128

OG3146

OG3116

OG3104

OG3122

OG3140

TEV

OG3130

OG3106

OG3124

OG3142

OG3112

OG3100

OG3118

OG3136

3C

OG3135

OG3111

OG3129

OG3147

OG3117

OG3105

OG3123

OG3141

-

OG3153

OG3150

OG3156

OG3155

OG3151

OG3148

OG3152

OG3154