Mammalian secretory tag properties and uses:

We offer several different mammalian secretory tags in easy-to-clone formats. The rationale for selling several different tags is because experience shows that no single tag is ideal for all proteins, and some proteins work better with different secretory tags.

Our product rangeMammTag

Oxford Genetics provides the following mammalian secretory tags:

  • BM-40 (osteonectin SPARC)
  • Vesicular Stomatitis Virus G (VSVG) protein
  • Chymotrypsinogen
  • Human interleukin-2 (IL-2)
  • Gaussia luciferase
  • Human serum albumin
  • Influenza haemagglutinin
  • Human insulin

Each of these proteins is provided in a vector allowing simple N-terminal fusion with your gene of interest. Most of the constructs also include puromycin resistance to enable simple selection of transfected cells.

The plasmid structure shown includes the insulin secretory tag upstream of the MCS, for positioning at the N terminus of your gene of interest, all driven by the CMV promoter. The construct also contains puromycin resistance driven by the ubiquitin promoter, for selection of transfected mammalian cells.

Simple secretory tag plasmids for use in mammalian cells

Tagz

BM40

VSVG

Chymotrypsinogen

Human IL-2

Product code

OG1494

OG1495

OG1496

OG1497

Tag

Gaussia luc

Albumin

Influenza Haemagglutinin

Human insulin

Product code

OG1498

OG1499

OG1500

OG1436

We also provide the insulin secretory peptide and the BM-40 secretory peptides in more sophisticated formats, providing the world's largest range of combinations of signal peptides with additional functional tags and protease cleavage sites:

BM-40 (osteonectin SPARC) and Insulin secretory signal peptides; we sell an extensive range of plasmids containing these signal peptides together with various peptide tags and protease-cleavage sites. In each case the secretory signal peptide will be naturally removed during secretion and the protease cleavage site allows removal of the tag from the protein if/when required after production.

We provide these secretory signal peptides with many different epitope or affinity tags

-          Epitope tags: V5, S-Tag, C-Myc, FLAG, Haemagglutinin (HA), T7

-          Affinity tags: Streptavidin, hexahistidine

Combined with a broad range of protease cleavage sites:

-          Factor Xa – cleavage specificity IEGR?

-          enterokinase (EKT) - cleavage specificity DDDDK

-          3C Prescission – cleavage specificity LEVLFQ?GP

-          Thrombin – cleavage specificity LVPR?GS

-          TEV (Tobacco Etch Virus) – cleavage specificity ENLYFQG

Secretory tag plasmids with additional functional tags, for use in mammalian cells

Secretory Tag: Insulin

Functional Tag

T7

V5

FLAG

Strep

C-Myc

6-His

HA

S-Tag

 

 

Protease Cleavage Site

EKT

OG1410

OG1386

OG1404

OG1422

OG1392

OG1380

OG1398

OG1416

Thr

OG1412

OG1388

OG1406

OG1424

OG1394

OG1382

OG1400

OG1418

FXa

OG1413

OG1389

OG1407

OG1425

OG1395

OG1383

OG1401

OG1419

TEV

OG1409

OG1385

OG1403

OG1421

OG1391

OG1379

OG1397

OG1415

3C

OG1414

OG1390

OG1408

OG1426

OG1396

OG1384

OG1402

OG1420

-

OG1432

OG1429

OG1435

OG1434

OG1430

OG1427

OG1431

OG1433

Secretory Tag: BM40

Functional Tag

T7

V5

FLAG

Strep

C-Myc

6-His

HA

S-Tag

 

Protease Cleavage Site

EKT

OG1468

OG1444

OG1462

OG1480

OG1450

OG1438

OG1456

OG1474

Thr

OG1470

OG1446

OG1464

OG1482

OG1452

OG1440

OG1458

OG1476

FXa

OG1471

OG1447

OG1465

OG1483

OG1453

OG1441

OG1459

OG1477

TEV

OG1467

OG1443

OG1461

OG1479

OG1449

OG1437

OG1455

OG1473

3C

OG1472

OG1448

OG1466

OG1484

OG1454

OG1442

OG1460

OG1478

-

OG1490

OG1487

OG1493

OG1492

OG1488

OG1485

OG1489

OG1491

 

For simplicity, all of our tags are positioned upstream of the MCS and will be included at the N terminal of your inserted protein sequence. This should be sufficient for all needs, however do contact us if you require C terminal tags, as these are very easy for us to provide.