Yeast secretory tag properties and uses:

We offer several different secretory tags for use in yeast cells, in easy-to-clone formats. The rationale for selling several different tags is because experience shows that no single tag is ideal for all proteins, and some proteins work better with different secretory tags.

Our product range

Oxford Genetics provides the following secretory tags for use in yeast cells:Yeastsecret

  • Inulase
  • Invertase
  • Killer protein
  • Lysozyme
  • Albumin
  • Alpha amylase
  • Alpha factor (full length)
  • Alpha factor (secretory peptide)
  • Glucoamylase

Each of these secretory peptides is provided in a vector allowing simple N-terminal fusion with your gene of interest. Most of the constructs also include the auxotrophic Uracil selection expression cassette URA3 gene, allowing positive selection of transfected yeast cells by growing the population in the absence of uracil. The plasmid structure shown contains the Glucoamylase secretory tag positioned upstream of the MCS, for inclusion at the N terminal of your gene of interest.

 

Simple secretory tag plasmids for use in yeast cells

Tag

Inulase

Invertase

Killer protein

Lysozyme

Albumin

Product code

OG1878

OG1879

OG1880

OG1881

OG1882

Tag

Alpha amylase

Alpha Factor (full length)

Alpha Factor (secretory peptide)

Gluco

amylase

 

Product code

OG1817

OG1875

OG1876

OG1877

 

 

We also provide the alpha amylase secretory peptide and the full length alpha factor in a comprehensive range of formats to meet all your requirements:

Alpha amylase signal peptide and the full length alpha factor; we sell an extensive range of plasmids containing these signal peptides together with a range of additional functional peptide tags and protease-cleavage sites. In each case the secretory signal peptide will be naturally removed during secretion and the protease cleavage site allows removal of the additional functional tag from the protein if/when required after production.

 

We provide these secretory signal peptides with many different epitope or affinity tags

-          Epitope tags: V5, S-Tag, C-Myc, FLAG, Haemagglutinin (HA), T7

-          Affinity tags: streptavidin, hexahistidine

 

Combined with a broad range of enzyme cleavage sites:

-          Factor Xa – cleavage specificity IEGR?

-          Enterokinase (EKT) - cleavage specificity DDDDK

-          3C Prescission – cleavage specificity LEVLFQ?GP

-          Thrombin – cleavage specificity LVPR?GS

-          TEV (Tobacco Etch Virus) – cleavage specificity ENLYFQG
 

For simplicity, all of our tags are positioned upstream of the MCS and will be included at the N terminal of your inserted protein sequence. This should be sufficient for all needs, however do contact us if you require C terminal tags, as these are very easy for us to provide.

 

Secretory tag plasmids with additional functional tags, for use in yeast cells

Secretory Tag: Alpha amylase

Functional Tag

T7

V5

FLAG

Strep

C-Myc

6-His

HA

S-Tag

 

 

Protease Cleavage Site

EKT

OG1791

OG1767

OG1785

OG1803

OG1773

OG1761

OG1779

OG1797

Thr

OG1793

OG1769

OG1787

OG1805

OG1775

OG1763

OG1781

OG1799

FXa

OG1794

OG1770

OG1788

OG1806

OG1776

OG1764

OG1782

OG1800

TEV

OG1790

OG1766

OG1784

OG1802

OG1772

OG1760

OG1778

OG1796

3C

OG1795

OG1771

OG1789

OG1807

OG1777

OG1765

OG1783

OG1801

-

OG1813

OG1810

OG1816

OG1815

OG1811

OG1808

OG1812

OG1814

Secretory Tag: Alpha factor

Functional Tag

T7

V5

FLAG

Strep

C-Myc

6-His

HA

S-Tag

 

Protease Cleavage Site

EKT

OG1849

OG1825

OG1843

OG1861

OG1831

OG1819

OG1837

OG1855

Thr

OG1851

OG1827

OG1845

OG1863

OG1833

OG1821

OG1839

OG1857

FXa

OG1852

OG1828

OG1846

OG1864

OG1834

OG1822

OG1840

OG1858

TEV

OG1848

OG1824

OG1842

OG1860

OG1830

OG1818

OG1836

OG1854

3C

OG1853

OG1829

OG1847

OG1865

OG1835

OG1823

OG1841

OG1859

-

OG1871

OG1868

OG1874

OG1873

OG1869

OG1866

OG1870

OG1872