Plasmid Preparation

We can provide highly pure plasmid DNA preparations ranging from miniprep (5 µg) to gigaprep (7.5 mg). 

 

Plasmid Quality Control

All of our plasmid undergo full UV spectral analysis and only DNA preparations passing our stringent QC analysis are released to customers. The purified plasmid preparation will also be analysed by gel electrophoresis and only preps showing >90% supercoiled DNA pass quality control.

 

UV Spectral QC Cut-offs

260:280 Ratio: 1.8-1.9

260:230 Ratio: >2.1 (typically 2.1-2.3)

Typical DNA Preparation: All plasmids are subject to full spectral QC and must pass both 260/280 and 260/230 ratios as well as demonstrating >90% supercoiled DNA by electrophoresis.

 

Free Sequencing and Digest

If the plasmid is based on our plasmid backbone we can sequence any region on request using primers OGP-F1-F10 or OGP-R1-R10 free of charge (not available for miniprep scale). We can also perform a test digest on any vector required free of charge if required.

 

Guaranteed Plasmid Yield

We can guarantee the yield for each of our own plasmids when purchasing the preps above, unless it is one of our low copy origin plasmids, or a plasmid we know is difficult to prepare (pSF-AAV-2 for example). If you have requested us to bulk prepare one of your plasmids, we will perform an initial miniprep of the plasmid to determine if it is likely that the scale you have purchased is achievable. If it is clear that plasmid yield is lower than expected we will contact you before proceeding and no charges will be applied if you choose not to proceed. 

 

Scales and purity levels provided:

Minimum Provided

Product Code

150 µg  OG-Midi
150 µg  OG-Midi-EDTX
500 µg  OG-Maxi
500 µg  OG-Maxi-EDTX
2 mg OG-Mega
2 mg OG-Mega-EDTX
7.5 mg  OG-Giga
7.5 mg  OG-Giga-EDTX

 

Yields typically exceed the above estimates, but we try to guarantee the amount shown where possible. DNA is provided in either TE (10mM Tris-HCl pH 8.0, 0.1mM EDTA), H2O, or 10mM Tris-HCl pH 8.0 at the customer’s request. Please note that EDTA can interfere with sequencing reactions.