Synthetic Biology is a rapidly emerging and exciting new discipline, and relies on complex genetic engineering strategies to build new biological systems that push the boundaries of genetics research. In order to reduce the variability in the DNA we build, we have developed a standardised DNA plasmid engineering platform called SnapFast™, which contains thousands of DNA sections (e.g. promoters, UTRs, gene) in configurations that allow them to be easily moved from one vector to another and create complex expression systems that maximise yields.
All of our constructs have been pre-designed to work together, and our expert team of engineers are here to help you create the vectors you need for your research and development projects. Where possible industry standard sequences have been optimised to provide efficient protein expression and simplified DNA assembly designs.
We have one of the largest collections of inter-compatible plasmids available globally, providing multiple expression and cloning options for almost every required permuation you might need. Our product range includes:
|Design of Cloning Strategies||Easy^||Medium||Easy||Hard*||Hard*||Hard*||hard*|
|Seamless Fusion with Tags||Yes||No||No||Yes||Yes||yes||Yes|
|Cloning Methods Patented||No||Yes||Yes||Yes||No||Yes||Yes|
|License Required for Commercial Research||No||Yes**||Yes**||No||No||No||No|
|Number of Plasmids||>1650||<100||<20||N/A||N/A||N/A||<200|
|Unique Cloning Positions in every plasmid||>20||1||1||N/A||N/A||N/A||1|
|Entry Vectors required||No||Yes||Yes||No||No||No||Yes|
|Tied into one cloning system?||No||Yes||Yes||No||No||No||Yes|
|Sequencing Primers||20 - complete coverage||2||2||N/A||N/A||N/A||
|Sequences Provided Online||Yes||Yes||Yes||N/A||N/A||N/A||
* Requires primer design using homology or TypeIIS sites
** Patents restrict the use of the cloning technology or products
^ Tools available online to help with design
LIC = Ligase Independent Cloning
Synthetic biology is an emerging field that is set to change the way scientists work with DNA. Our plasmids are uniquely designed to allow the creation of complex DNA expression vectors with minimal cloning steps. This feature makes them the ideal tool set for synthetic biology applications and enables us to offer versatile cloning systems that deliver results, regardless of a projects complexity.
Whilst most of the DNA sections within our plasmids are flanked by restriction sites of 6 base pairs (bp) in length, we have also strategically placed 8 bp restriction sites at key positions throughout the plasmid to maximise the versatility of the system. The low frequency of each 8 bp cutting site in random DNA makes it unlikely that any gene you insert will contain such a site. By exploiting this principle our DNA plasmids have limitless potential and unparalled flexibility.
Benefits of the SnapFastTM system:
If you would like to learn more about our system, or you have a specific question about one of our products, please contact us for further information.