This pack allows you to determine the optimum origin of replication for your bacterial experimental/cloning system. Four different bacterial origins are provided, each providing different plasmid copy number in bacterial cells. In general, low copy origins are used for very large plasmids, or where leakage of mammalian transgene expression may be toxic to the bacteria. The CMV promoter is available in each of these plasmids, to regulate expression in mammalian cells of any gene you insert into the MCS.
This kit contains the plasmids pSF-CMV-SC101 (OG13), pSF-CMV-BR322 (OG12), pSF-CMV-Kan (OG10) and pSF-CMV-p15A (OG11).