This pack allows you to compare the activity of eleven different bacterial secretory tags (signal peptides) to identify which is most efficient for the secretion of your protein of interest. This can be very important in influencing yields in bacterial cultures. The most efficient tag seems to depend on the protein of interest and also on the cells used, hence we consider it important to compare several tags in order to select the best. Inserting your gene of interest into the MCS of these plasmids will place it downstream of the signal peptide, under regulatory control of the strong constitutive OXB20 bacterial promoter.
This kit contains the plasmids pSF-OXB20-NH2-OmpA (OG3099), pSF-OXB20-NH2-PelB (OG3157), pSF-OXB20-NH2-dsbA (OG3158), pSF-OXB20-NH2-gIII (OG3159), pSF-OXB20-NH2-OmpC (OG3160), pSF-OXB20-NH2-OmpT (OG3161), pSF-OXB20-NH2-PhoA (OG3162), pSF-OXB20-NH2-SufI (OG3163), pSF-OXB20-NH2-TorA (OG3164), pSF-OXB20-NH2-TorT (OG3165) and pSF-OXB20-NH2-MalE (OG3166).