This pack enables you to compare placing decahistidine (10His) affinity tags at either the N or C terminus of your gene of interest (inserted into the MCS, under transcriptional control of the CMV promoter) with, and also without a TEV (Tobacco Etch Virus) protease cleavage site. The TEV site enables removal of the 10His tag from the protein following purification. Comparing these four configurations should enable you to evaluate how best to express and purify your gene of interest from mammalian cells. We also provide many other functional tags and cleavage sites, if required.
This kit contains the plasmids pSF-CMV-Puro-NH2-His10 (OG3210), pSF-CMV-Puro-COOH-TEV-10His (OG1120), pSF-CMV-Puro-COOH-10His (OG3420) and pSF-CMV-Puro-NH2-10His-TEV (OG1119).