pSF-OXB20-SnapFusion (OG605) Bacterial SnapFusion Constiutive Plasmid

Product Code: OG605R1
Product Code: OG605C1
Product Code: OG605G1

Product Description

Bacterial vector designed for the insertion of genes that have been configured for fusion peptide tag range, but in this plasmid there is no tag to allow normal un-taggged expression.

Plasmid Information

Product Name: pSF-OXB20-SnapFusion

Product Code: OG605

Size (bp): 3866 bp

Bacterial Antibiotic Selection: KanR

Origin and Compatibility: pUC high copy derived from pBR322

Bacterial Copy Number: 500-700 per cell

Promoter: OXB20 strong constitutive bacterial promoter

Plasmid Purpose:

This vector is a standard E.coli expression plasmid with the exception that it has been modified to allow the direct cloning of gene fragments that have been designed to fit with any of our peptide tag plasmid range. This can be useful if you have created a peptide fusion but you would also like to create a non-tagged gene variant. The multiple cloning site contains two BsgI and BseRI sites that produce overhangs that are compatible with gene designed using our SnapFusion primer designer (see the cloning tab for this product). The promoter in this plasmid is a strong constitutive bacterial promoter that does not require induction for activity.

Promoter Expression Level:

This plasmid contains a very strong constitutive E.coli promoter that was derived from the RecA promoter by removing the LexA repressor site. It is part of our constitutive bacterial promoter range. This promoter shows the highest level of expression in the promoter range with OXB1 showing the lowest level. They require no inducing agent for expression.

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Transcription Termination:

This plasmid contains three alternative transcription terminators for mammalian bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.

Intellectual Property Status

This product is part of our SnapFastâ„¢ plasmid range, for more information on the intellectual property status of this plasmid please click here. For more information on the terms of our licences please click here.