Encephalomyocarditis virus (EMCV) IRES plasmid for expressing wild-type Streptococcus pyogenes Cas9 protein in mammalian cells together with the fluorescent marker EGFP. The Cas9 gene has been inserted into the main SnapFast™ multiple cloning site (MCS). Expression is driven by the EF1 alpha promoter. The plasmid also contains a site for insertion of a guide RNA sequence.
Product Name: pSF-EF1-Alpha-Cas9WT-EMCV-GFP-gRNA (SnapFast Pro™)
Product Code: OG5209
Size (bp): 10738 bp
Bacterial Antibiotic Selection: KanR
Origin and Compatibility: pUC high copy derived from pBR322
Bacterial Copy Number: 500-700 per cell
Promoter: Human Elongation Factor Alpha Promoter
This plasmid is designed for expressing wild-type Streptococcus pyogenes Cas9 protein in mammalian cells. Cas9 is the DNA endonuclease central to the CRISPR genome editing system. Expression of Cas9 is driven by the EF1 alpha promoter.
CRISPR genome editing requires a guide RNA sequence to direct Cas9 to a particular DNA sequence. This plasmid also contains a site for insertion of a guide RNA sequence. This plasmid is also compatible with the double gRNA adaptor (OG3519) which allows insertion of 2 gRNAs instead of 1. For more information read our guide to CRISPR technologies or view a complete list of our CRISPR plasmids.
This product is part of our SnapFast Pro™ plasmid range, for more information on the intellectual property status of this plasmid and the terms of our licences please click here.