Encephalomyocarditis virus (EMCV) IRES plasmid for expressing Cas9 together with a fluorescent EGFP marker. This plasmid contains the major immediate early cytomegalovirus (CMV) enhancer region to allows the insertion and enhancement of weaker promoters into the promoter multiple cloning site.
Product Name: pSF-pA-CMVe-Cas9WT-EMCV-eGFP (SnapFast Pro™)
Product Code: OG4761
Size (bp): 9743 bp
Bacterial Antibiotic Selection: KanR
Origin and Compatibility: pUC high copy derived from pBR322
Bacterial Copy Number: 500-700 per cell
This plasmid is designed for expressing wild-type Streptococcus pyogenes Cas9 protein in mammalian cells. Cas9 is the DNA endonuclease central to the CRISPR genome editing system. For more information about CRISPR read our guide to CRISPR technologies or view a complete list of our CRISPR plasmids.
This product is part of our SnapFast Pro™ plasmid range, for more information on the intellectual property status of this plasmid and the terms of our licences please click here.