Encephalomyocarditis virus (EMCV) IRES plasmid for expressing Cas9 together with the fluorescent marker EGFP. The plasmid also contains a multiple cloning site in the promoter position designed to allow you to insert your own promoter to drive the expression of Cas9.
Product Name: pSF-pA-PromMCS-Cas9WT-EMCV-eGFP (SnapFast Pro™)
Product Code: OG4759
Size (bp): 9251 bp
Bacterial Antibiotic Selection: KanR
Origin and Compatibility: pUC high copy derived from pBR322
Bacterial Copy Number: 500-700 per cell
This plasmid is designed for expressing wild-type Streptococcus pyogenes Cas9 protein in mammalian cells. Cas9 is the DNA endonuclease central to the CRISPR genome editing system. For more information on CRISPR gene editing read our guide to CRISPR technologies or view a complete list of our CRISPR plasmids.
This product is part of our SnapFast Pro™ plasmid range, for more information on the intellectual property status of this plasmid and the terms of our licences please click here.