Dual CAG promoter expression vector. This vector contains two separate expression cassette for expression of genes under the strong mammalian CAG promoter (formed from the CMV enhancer, chicken beta actin promoter and rabbit beta globin intron). The construct contains two separate MCS for inserting the genes of interest and two separate polyA signal for transcription termination in mammalian cells.
Product Name: pSF-CAG-MCS-pA-CAG-MCS-pA
Product Code: OG4116
Size (bp): 7362 bp
Bacterial Antibiotic Selection: KanR
Origin and Compatibility: pUC high copy derived from pBR322
Bacterial Copy Number: 500-700 per cell
Promoter: CAG synthetic mammalian promoter and Ubiquitin promoter / CAG synthetic mammalian promoter and Ubiquitin promoter / Chicken Beta Actin (CBA) promoter / Chicken Beta Actin (CBA) promoter
This mammalian expression vector contains two chimeric CAG promoter expression cassette with two unique MCS region for cloning your gene of interest. The CAG promoter is a composite of the CMV enhancer, the chicken beta actin promoter (CBA) and the rabbit beta globin intron. This promoter is often used instead of the CMV promoter because in some cell types the CMV promoter can get silenced. To prevent this the CAG promoter contains the CpG island from the CBA promoter to help prevent promoter methylation. The plasmid can be used to drive expression of up to two proteins in a range of mammalian cell types and we have consistently found that expression levels are equal to CMV in short term experiments in standard cell types.
Promoter Expression Level:
This plasmid contains the mammalian CAG promoter which is a synthetic composite of the CMV immediate early enhancer followed by the CBA promoter and the rabbit beta globin intron. The Chicken beta actin contains a CpG island that can help to keep the promoter active for longer in stable culture when compared to the CMV promoter.
This product is part of our SnapFast™ plasmid range, for more information on the intellectual property status of this plasmid and the terms of our licences please click here.