pSF-CMV-HuIgG1_HC (OG527) Human IgG Heavy Chain Plasmid (SnapFast Pro™)

Product Code: OG527R1
Product Code: OG527G1

Product Description

Human IgG heavy chain constant region expression vector where variable regions can be fused to the constant region to create your desired IgG heavy chain plasmid

Plasmid Information

Product Name: pSF-CMV-HuIgG1_HC (SnapFast Pro™)

Product Code: OG527

Size (bp): 5248 bp

Bacterial Antibiotic Selection: KanR

Origin and Compatibility: pUC high copy derived from pBR322

Bacterial Copy Number: 500-700 per cell

Promoter: Cytomegalovirus (CMV) immediate early promoter

Plasmid Purpose:

This expression plasmid contains the human IgG heavy chain constant region and has been designed to allow the seamless fusion of variable antibody fragments to create full length heavy chain expression constructs. The vector has been engineered to contain a BseRI restriction enzyme site upstream of the constant domain that when cleaved produces an overhang of the first two bases of the first codon of the constant region. This allows variable antibody fragments to be cloned upstream to create full length antibody genes. We also provide plasmids for human Kappa and Lambda light chain expression (termed pSF-CMV-HuKappa LC and pSF-CMV-HuLambda LC repectively). We also have a range of antibody expression plasmids for mouse and rat IgG heavy and light chains.

Promoter Expression Level:

This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. For this reason we stock a range of other promoters that are compatible with this plasmid and are available on request.

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Transcription Termination:

This plasmid contains three alternative transcription terminators for mammalian bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.

Intellectual Property Status

This product is part of our SnapFast Pro™ plasmid range, for more information on the intellectual property status of this plasmid please click here. For more information on the terms of our licences please click here.