Product Name: pSF-CMV-HuKappa_LC (SnapFast Pro™)
Product Code: OG528
Size (bp): 4579 bp
Bacterial Antibiotic Selection: KanR
Origin and Compatibility: pUC high copy derived from pBR322
Bacterial Copy Number: 500-700 per cell
Promoter: Cytomegalovirus (CMV) immediate early promoter
This is an antibody expression plasmid designed for the generation of full length human kappa light chain expression cassettes. The vector has been designed to contain a non-palindomic resitriction site called BseRI upstream from the light chain coding region. When this enzyme is used it produces an overhang from the first two two nucleotides in the first codon of the constant region. This allows seamless fusion to be made between variable regions carrying the same overhang. This can be achieved by creating a variable region PCR fragment that contains a BserRI site at the end in the opposing orientation.Promoter Expression Level:
This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. For this reason we stock a range of other promoters that are compatible with this plasmid and are available on request.
This plasmid contains three alternative transcription terminators for mammalian bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.