pSF-CMV-HuLambda_LC (OG529) Human Lambda Light Chain Antibody Vector (SnapFast Pro™)

Product Code: OG529R1
Product Code: OG529G1

Product Description

Light chain (Lambda) human antibody expression plasmid allowing the fusion of variable regions to the constant region (in this plasmid) for antibody expression

Plasmid Information

Product Name: pSF-CMV-HuLambda_LC (SnapFast Pro™)

Product Code: OG529

Size (bp): 4576 bp

Bacterial Antibiotic Selection: KanR

Origin and Compatibility: pUC high copy derived from pBR322

Bacterial Copy Number: 500-700 per cell

Promoter: Cytomegalovirus (CMV) immediate early promoter

Plasmid Purpose:

An antibody expression plasmid designed for the production of human antibody lambda genes by fusing variable antibody fragments with the constant domain in this plasmid. This can be achieved because the contstant region coding sequence in this plasmid is positioned downstream of a BseRI restriction site which when cleaved will produce an overhang consisting of the first two nucleotides of the first codon of the constant region. This allows variable antibody fragments to be seamlessly inserted by placing a BseRI site in the opposing direction at the end of the variable fragment.

We also provide plasmids for the expression of both rat and mouse antibodies as well as vectors for the expression of human IgG heavy chain and Kappa light chain (called pSF-CMV-HuIgG1 HC and pSF-CMV-HuKappa LC respectively)

Promoter Expression Level:

This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. For this reason we stock a range of other promoters that are compatible with this plasmid and are available on request.

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Transcription Termination:

This plasmid contains three alternative transcription terminators for mammalian bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.

Intellectual Property Status

This product is part of our SnapFast Pro™ plasmid range, for more information on the intellectual property status of this plasmid please click here. For more information on the terms of our licences please click here.