pSF-CMV-MuKappa (OG568) Mouse Kappa Light Chain Antibody Plasmid (SnapFast Pro™)

Product Code: OG568R1
Product Code: OG568G1

Product Description

Murine Kappa light chain constant region expression plasmid. Variable antibody fragments can be fused in this plasmid to create full length antibody light chains

Plasmid Information

Product Name: pSF-CMV-MuKappa (SnapFast Pro™)

Product Code: OG568

Size (bp): 4576 bp

Bacterial Antibiotic Selection: KanR

Origin and Compatibility: pUC high copy derived from pBR322

Bacterial Copy Number: 500-700 per cell

Promoter: Cytomegalovirus (CMV) immediate early promoter

Plasmid Purpose:

This expression plasmid encodes the constant region from the mouse kappa light chain antibody gene and is designed to allow the fusion of variable antibody gene segments seamlessly to create light chain cassettes. This is possible because there is a BseRI restriction enzyme site upstream of the kappa coding sequence that when cleaved produces an overhang from the first two nucleotides of the first codon of the constant region. By designing the variable regions of antibodies to have a BseRI site at the end in an opposing direction it is possible to seamlessly fuse the variable and constant domains together.

Promoter Expression Level:

This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. For this reason we stock a range of other promoters that are compatible with this plasmid and are available on request.

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Transcription Termination:

This plasmid contains three alternative transcription terminators for mammalian bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.

Intellectual Property Status

This product is part of our SnapFast Pro™ plasmid range, for more information on the intellectual property status of this plasmid please click here. For more information on the terms of our licences please click here.