Product Name: pSF-CMV-Rat-IgG1_HC (SnapFast Pro™)
Product Code: OG524
Size (bp): 5236 bp
Bacterial Antibiotic Selection: KanR
Origin and Compatibility: pUC high copy derived from pBR322
Bacterial Copy Number: 500-700 per cell
Promoter: Cytomegalovirus (CMV) immediate early promoter
This plasmid is designed for the generation and expression of heavy chain rat (Rattus rattus) immunoglobulin antibody genes. The vector has been formatted so that it can be cleaved to produce a restriction site overhang (using BseRI) within the first codon of the antibody constant region. This allows the variable regions of antibodies to be inserted to create full length heavy chain expression cassettes. As part of this product range we also have antibody vectors for the production of mouse and human antibodies as well as plasmids for the production of rat Kappa and Lambda antibody light chains (pSF-CMV-Rat-Kappa and pSF-CMV-Rat-Lambda respectively).Promoter Expression Level:
This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. For this reason we stock a range of other promoters that are compatible with this plasmid and are available on request.
This plasmid contains three alternative transcription terminators for mammalian bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.