pSF-CMV-Rat-IgG1_HC (OG524) Rat IgG1 Heavy Chain Antibody Plasmid (SnapFast Pro™)

Product Code: OG524R1
Product Code: OG524G1

Product Description

Rat IgG heavy chain constant region mammalian expression plasmid vector using the CMV promoter to control protein production. Variable regions can be fused to the heavy chain to create full length antibody heavy chains.

Plasmid Information

Product Name: pSF-CMV-Rat-IgG1_HC (SnapFast Pro™)

Product Code: OG524

Size (bp): 5236 bp

Bacterial Antibiotic Selection: KanR

Origin and Compatibility: pUC high copy derived from pBR322

Bacterial Copy Number: 500-700 per cell

Promoter: Cytomegalovirus (CMV) immediate early promoter

Plasmid Purpose:

This plasmid is designed for the generation and expression of heavy chain rat (Rattus rattus) immunoglobulin antibody genes. The vector has been formatted so that it can be cleaved to produce a restriction site overhang (using BseRI) within the first codon of the antibody constant region. This allows the variable regions of antibodies to be inserted to create full length heavy chain expression cassettes. As part of this product range we also have antibody vectors for the production of mouse and human antibodies as well as plasmids for the production of rat Kappa and Lambda antibody light chains (pSF-CMV-Rat-Kappa and pSF-CMV-Rat-Lambda respectively).

Promoter Expression Level:

This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. For this reason we stock a range of other promoters that are compatible with this plasmid and are available on request.

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Transcription Termination:

This plasmid contains three alternative transcription terminators for mammalian bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.

Intellectual Property Status

This product is part of our SnapFast Pro™ plasmid range, for more information on the intellectual property status of this plasmid please click here. For more information on the terms of our licences please click here.