pSF-CMV-Rat-Kappa_LC (OG525) Rat Kappa Light Chain Antibody Plasmid (SnapFast Pro™)

Product Code: OG525R1
Product Code: OG525G1

Product Description

Rat Kappa light chain expression plasmid where the constant region of the rat light chain can be fused to variable regions to create full length light chain genes.

Plasmid Information

Product Name: pSF-CMV-Rat-Kappa_LC (SnapFast Pro™)

Product Code: OG525

Size (bp): 4576 bp

Bacterial Antibiotic Selection: KanR

Origin and Compatibility: pUC high copy derived from pBR322

Bacterial Copy Number: 500-700 per cell

Promoter: Cytomegalovirus (CMV) immediate early promoter

Plasmid Purpose:

This vector is for the production of kappa antibody light chains for rat (Rattus rattus). It has been engineered so that when the vector is cut with the restriction enzyme BseRI it produces an overhang from the first codon of the constant region. This allows antibody light chain variable regions to be seamlessly ligated into this vector to create full length rat kappa light chain antibody expression cassettes. As part of this vector range we also provide plasmids for the production of both human and mouse antibodies as well as vectors for the creation of Heavy IgG chain and Lambda light chain rat antibodies (named pSF-CMV-Rat-IgG HC and pSF-CMV-Rat-Lambda respectively)

Promoter Expression Level:

This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. For this reason we stock a range of other promoters that are compatible with this plasmid and are available on request.

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Transcription Termination:

This plasmid contains three alternative transcription terminators for mammalian bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.

Intellectual Property Status

This product is part of our SnapFast Pro™ plasmid range, for more information on the intellectual property status of this plasmid please click here. For more information on the terms of our licences please click here.