Product Name: pSF-CMV-TOPO
Product Code: OG108
Size (bp): 4343 bp
Bacterial Antibiotic Selection: AmpR
Origin and Compatibility: pUC high copy derived from pBR322
Bacterial Copy Number: 500-700 per cell
Promoter: Cytomegalovirus (CMV) immediate early promoter
A versatile cloning plasmid for the expression of genes in mammalian cells. This plasmid contains the multiple cloning site (MCS) from Life Technologies TOPO vector however it has been modified slightly to accommodate some restriction sites in the SnapFastTM system. These changes are described in the full plasmid details PDF. The use of this MCS instead of the normal SnapFastTM vector MCS will limit the ability to use some of the inserts that we sell that immediately flank or are inserted within the standard MCS. In this vector these limitations mainly extend to NcoI aligned peptide tags and signal peptides however these same inserts can still be ligated into the NotI and HindIII sites in this MCS. Most other inserts should still be compatible with this plasmid. TOPO is a registered trademark of Life Technologies.Promoter Expression Level:
This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. For this reason we stock a range of other promoters that are compatible with this plasmid and are available on request.
This plasmid contains three alternative transcription terminators for mammalian bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.
This vector contains a different multiple cloning site (MCS) from the standard SnapFastTM vector. The MCS is derived from TOPO. The order of the restriction sites in this vector are almost the same as in TOPO however the sequence is not. The TOPO MCS has also been modified as follows: