pSF-CMV-pUC19 (OG106) CMV pUC19 MCS Plasmid

Product Code: OG106R1
Product Code: OG106C1
Product Code: OG106G1

Product Description

pUC19 multiple cloning site CMV plasmid. The vector is modified to contain the restriction enzyme pattern from the cloning plasmid pUC19.

Plasmid Information

Product Name: pSF-CMV-pUC19

Product Code: OG106

Size (bp): 4309 bp

Bacterial Antibiotic Selection: AmpR

Origin and Compatibility: pUC high copy derived from pBR322

Bacterial Copy Number: 500-700 per cell

Promoter: Cytomegalovirus (CMV) immediate early promoter

Plasmid Purpose:

A versatile cloning plasmid for the expression of genes in mammalian cells. This plasmid contains the multiple cloning site (MCS) from pUC19 however it has been modified slightly to accommodate some restriction sites in the SnapFastTM system. These changes are described in the full plasmid details PDF. The use of this MCS instead of the normal SnapFastTM vector MCS will limit the ability to use some of the inserts that we sell that immediately flank or are inserted within the standard MCS. This primarily includes N-terminal tags and signal peptides. This is because these inserts are flanked by restriction sites that are not compatible with the pUC19 MCS. Most other inserts should still be compatible with this plasmid.

Promoter Expression Level:

This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. For this reason we stock a range of other promoters that are compatible with this plasmid and are available on request.

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Transcription Termination:

This plasmid contains three alternative transcription terminators for mammalian bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.

Multiple cloning site notes:

This vector contains a different multiple cloning site (MCS) from our standard vectors. The MCS is derived from pUC19. The order of the restriction sites in this vector are almost the same as in pUC19 however the sequence is not. The pUC19 MCS has also been modified as follows.

  • The XbaI site in the pUC19 MCS has been replaced with a SpeI site (these sites produce compatible cohesive ends when cleaved).
  • The XbaI site in the pUC19 MCS has been replaced with a SpeI site (these sites produce compatible cohesive ends when cleaved).
  • The SbfI and PstI sites have been replaced with an NsiI site (which produces compatible cohesive ends with SbfI and PstI when cleaved).
  • The BamHI site that is normally found in all SnapFast vectors (in the downstream fusion MCS) has been replaced with a BclI site because the pUC19 MCS contains this site. BamHI and BclI produce compatible cohesive ends when cleaved although BclI is methylated in this vector and will require growth in a Dam methylase negative bacterial strain (such as JM110 cells) before it can be used.
  • The ClaI to NheI sites have other functions such as adding peptide tags or IRES elements. The BsgI and BseRI restriction sites cleave within the stop codon in the XbaI site and allow the retrospective fusion of coding sequences. These sites are normally only used on genes that we sell in the main multiple cloning site.

Intellectual Property Status

This product is part of our SnapFastâ„¢ plasmid range, for more information on the intellectual property status of this plasmid please click here. For more information on the terms of our licences please click here.