pSF-H1 (OG52) siRNA/RNAi Expression Plasmid

Price: $253.91
Product code: OG52

Product Description

shRNA expression plasmid using the H1 RNA polymerase I promoter to drive transcription. Used to generate short hairpin RNA to silence genes.

Plasmid Information

Product Name: pSF-H1

Product Code: OG52

Size (bp): 3801 bp

Bacterial Antibiotic Selection: KanR

Origin and Compatibility: pUC high copy derived from pBR322

Bacterial Copy Number: 500-700 per cell

Promoter: Human H1 promoter

Plasmid Purpose:

The expression of short hairpin RNA in eukaryotic cells. The H1 is transcribed by RNA polymerase III (RNA pol III) which terminates transcription at a string of thymidine residues typically a minimum of five residues in mammalian cells. Unlike most SnapFastTM vectors this plasmid contains an RNA pol III terminator downstream of the XhoI site although it is more common to insert a terminator sequence at the end of the sequence you insert into the MCS that encodes a shRNA.

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Transcription Termination:

This vector contains an RNA polymerase III promoter (H1) which will terminate at a string of thymidine residues. Typically this is 5-6 T residues in length. This plasmid contains a string of thymidines in the multiple cloning site to allow termination.

Multiple cloning site notes:

This vector has a modified MCS compared to standard plasmids. The ATG start codon in the NcoI site has been removed as have the Shine-Dalgarno and KOZAK sequences as these are not required for RNA Pol3 expressed transcripts.

The terminator for RNA polymerase III has been positioned immediately downstream of the XhoI site. There is an additional string of Thymidine residues (the terminator sequence for RNA Pol III) at the end of the SV40 polyA region however using this site will produce a long transcript and has not been validated. We recommend inserting your hairpin DNA sequence between Hind3 and XhoI. The theoretical RNA position +1 is the first base of the NotI restriction site.

Intellectual Property Status

This product is part of our SnapFast plasmid range, for more information on the Intellectual property status of this plasmid please click here