Product Name: pSF-pA-MinProm-SEAP
Product Code: OG248
Size (bp): 5572 bp
Bacterial Antibiotic Selection: KanR
Origin and Compatibility: pUC high copy derived from pBR322
Bacterial Copy Number: 500-700 per cell
Promoter: HSV TK Minimal Promoter
This plasmid contains a multiple cloning site that is upstream of a minimal promoter that is upstream of the human secreted alkaline phosphatase (SEAP) reporter gene. This plasmid can be used to insert specific DNA sequences that will endow the minimal promoter with specific biochemical properties such as tissue specificity or transcription factor specific activation.
This plasmid contains three alternative transcription terminators for mammalian bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.
There is a start codon in the NcoI site in the MCS that can be removed by digestion with KpnI if required. The MCS for gene insertions extends from NotI to XbaI but there are built in Shine-Dalgarno and Kozak ribosomal entry site sequences aligned with the start codon in the NcoI site. The ClaI and NheI sites have other functions and allow the insertion of sequences from our other catalogue plasmids (such as IRES sequences and second promoters).
The BsgI and BseRI restriction sites in the plamsid cleave within the stop codon in the XbaI site in the MCS. This can allow retrospective fusion of coding sequences to our peptide tag range if your genes stop codon is positioned here.