pSF-Renilla-Photinus (OG595) Bi-directional Firefly Renilla Luciferase Plasmid

Product Code: OG595R1
Product Code: OG595C1
Product Code: OG595G1

Product Description

Dual reporter plasmid where Firefly luciferase is in an inverted orientation in relation to Renilla luciferase. Allowing the measurement of both reporters from one plasmid.

Plasmid Information

Product Name: pSF-Renilla-Photinus

Product Code: OG595

Size (bp): 6170 bp

Bacterial Antibiotic Selection: AmpR

Origin and Compatibility: pUC high copy derived from pBR322

Bacterial Copy Number: 500-700 per cell


Plasmid Purpose:

This plasmid has been designed so that sequences can inserted between two reporter genes (renilla and firefly luciferase) to allow the relative transcription level in both directions to be determined by measuring the reporter luminescence. Dual luciferase reporter assay kits are available from other providers that are compatible with this plasmid. The plasmid only contains a Bgl2 site between the two genes to allow insertion.

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Transcription Termination:

This plasmid contains three alternative transcription terminators for mammalian bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.

Intellectual Property Status

This product is part of our SnapFastâ„¢ plasmid range, for more information on the intellectual property status of this plasmid please click here. For more information on the terms of our licences please click here.