Product Name: pSF-Ad5-WT (SnapFast Pro™)
Product Code: OG617
Size (bp): 38870 bp
Bacterial Antibiotic Selection:
Origin and Compatibility:
Bacterial Copy Number:
Promoter: Adenovirus Serotype 5 E1A Promoter
This is a serotype 5 adenovirus virus plasmid vector that allows for the production of replicating wild-type adenoviruses. The vector contains the complete adenovirus serotype 5 genome cloned into a plasmid backbone with the kanamycin antibiotic selection that allows for the propagation of the vector in E. coli.
Recovery and Growth:
Virus recovery requires two steps firstly the plasmid must be cleaved with the PacI restriction enzyme to linearise the virus genome. Then the linear genome can be recovered and grown in mammalian. A typical recovery transfection would use approximately 5ug of DNA. It is not necessary to remove the plasmid backbone after linearising the DNA a standard DNA clean up will be sufficient rather than gel extraction. Virus colonies should appear approximately 10-15 days after transfection into 293 cells however clones can sometimes appear at earlier time points. Cell should be cultured in 2% fetal calf serum to prevent overgrowth during this time
This product is part of our SnapFast Pro™ plasmid range, for more information on the intellectual property status of this plasmid and the terms of our licences please click here.