Yeast vector designed for the insertion of genes that have been configured for fusion peptide tag range, but in this plasmid there is no tag to allow normal un-taggged expression.
Product Name: pSF-TEF1-SnapFusion
Product Code: OG604
Size (bp): 6729 bp
Bacterial Antibiotic Selection: KanR
Origin and Compatibility: pUC high copy derived from pBR322
Bacterial Copy Number: 500-700 per cell
Promoter: Yeast Elongation Factor Alpha-1 (TEF-1) promoter
This vector is a standard Saccharomyces cerevisiae yeast expression plasmid with the exception that it has been modified to allow the direct cloning of gene fragments that have been designed to fit with any of our peptide tag plasmid range. This can be useful if you have created a peptide fusion but you would also like to create a non-tagged gene variant. The multiple cloning site contains two BsgI and BseRI sites that produce overhangs that are compatible with gene designed using our SnapFusion primer designer (see the cloning tab for this product). The promoter in this plasmid (TEF1) is a strong constitutive yeast promoter.Promoter Expression Level:
This plasmid contains the yeast translation elongation factor 1 promoter. It is the strongest promoter that we provide for expression in Saccharomyces cerevisiae.
This plasmid contains three alternative transcription terminators for yeast bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.