Antibody Engineering & Production Services

 

At Oxford Genetics we offer a range of ultra-high-throughput antibody production and biotherapeutic development services including

Novel Bioinformatic Antibody Platform 

-Optimize codon sequences

-Assess sequence design using an in silico binding prediction algorithm

-Design humanized sequences

Antibody Production

-Robotics platforms drive ultra-high-throughput antibody production 

-Mammalian cell line expression (CHO and HEK)

-Multiple antibody formats (full length mAb, chimeric mAb, humanized mAb, Fab/ScFv)

Antibody Humanization

-Proprietary software for antibody humanization including immunogenicity filter providing a crucial stage in biotherapeutic development.

 

Key benefits  

State of the Art Automation - Our robotics platforms have been specifically designed to overcome the bottlenecks in antibody production providing a more uniform process with a quicker turnaround.

 

Antibody Production

Here is a typical timeline for an antibody project.

 

 

High Throughput Antibody Expression and Production

Scale

Up to 5 mg

Up to 10 mg

Up to 20 mg

Up to 30 mg

Up to 50mg

Purification

Protein A or G

Protein A or G

Protein A or G

Protein A or G

Protein A or G

Analytics

SDS-PAGE

ELISA

HPLC

SDS-PAGE

ELISA

HPLC

SDS-PAGE

ELISA

HPLC

SDS-PAGE

ELISA

HPLC

SDS-PAGE

ELISA

HPLC

Turnaround Time

2 to 3 weeks

2 to 3 weeks

2 to 3 weeks

2 to 3 weeks

2 to 3 weeks

Cell Line

HEK293 EBNA

HEK293 EBNA

HEK293 EBNA

HEK293 EBNA

HEK293 EBNA

 

On a case by case basis we can produce gram quantities of individual antibodies in mammalian cell lines e.g. HEK293 EBNA and CHO. This can be coupled with our cell line development and process development services.

 

 

Antibody Humanization

Project Design

Bioinformatics

Synthesis

Production

Analytics

Lead Time

-Statement of work generated

-Customer supplied variable region cloned and sequenced  

-Propriety algorithms design humanized sequences.

-Humanised sequence analysed using in silico binding prediction algorithm.

High fidelity synthesis of humanized sequence of variable region

-Chimeric antibody produced and validated

-Humanized variable regions are cloned into Oxford Genetics Propriety Plasmid creating fully humanised antibody.

-Using our bespoke robotics platforms enables an ultra-high throughput approach to cloning, DNA purification, cell culture and antibody purification.

SDS-PAGE, WB, ELISA,

HPLC, SPR

Approximately 24 weeks for
complete service offering. 

 

 

 

Additional Services

Cell Line Engineering

Oxford Genetics has expertise engineering mammalian cell lines using a variety of technologies including gene editing

 

CRISPR Cell Line Development

Oxford Genetics has expertise generating stable, recombinant, cell lines expressing heterologous products, such as proteins or viruses.